Class: Robot::Generator::TecanV3

Inherits:
TecanV2 show all
Includes:
Behaviours::TecanDefault
Defined in:
app/models/robot/generator/tecan_v3.rb

Overview

Handles picking file generation for Tecan robots with reusing tips for buffer addition steps

Constant Summary

Constants inherited from TecanV2

Robot::Generator::TecanV2::NUM_BUFFER_CHANNELS

Instance Attribute Summary

Attributes inherited from Base

#batch, #ctrl_barcode_index, #dest_barcode_index, #picking_data, #plate_barcode, #source_barcode_index

Instance Method Summary collapse

Methods inherited from TecanV2

#as_text, #filename

Methods inherited from Base

#initialize, #total_volume, #type

Constructor Details

This class inherits a constructor from Robot::Generator::Base

Instance Method Details

#buffer_separator ⇒ String

Adds a 'Break' command between the buffer and sample addition steps.

Returns:

  • (String) —

    the buffer separator string

See Also:

  • Robot::Generator::TecanV2#buffer_separator


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# File 'app/models/robot/generator/tecan_v3.rb', line 47

def buffer_separator
  'B;'
end

#buffers(data_object) ⇒ String

Groups buffer addition steps by channel, adds 'Comment' lines between steps, appends a 'Wash' command at the end of each channel group. This enables reusing tips for buffer addition steps within each channel.

rubocop:disable Metrics/AbcSize,Metrics/MethodLength

Parameters:

  • data_object (Hash) —

    the picking data object

Returns:

  • (String) —

    the buffer addition steps string

See Also:

  • Behaviours::TecanDefault#buffers


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# File 'app/models/robot/generator/tecan_v3.rb', line 16

def buffers(data_object)
  groups = Hash.new { |h, k| h[k] = [] } # channel => [steps]
  each_mapping(data_object) do |mapping, dest_plate_barcode, plate_details|
    next unless total_volume > mapping['volume']

    dest_name = data_object['destination'][dest_plate_barcode]['name']
    volume = mapping['buffer_volume']
    vert_map_id = description_to_column_index(mapping['dst_well'], plate_details['plate_size'])
    channel = channel_number(vert_map_id)

    # No Wash after each step, as we are reusing tips for each channel.
    step = <<~TECAN.chomp
      A;#{buffer_info(vert_map_id)};;#{tecan_precision_value(volume)}
      D;#{dest_plate_barcode};;#{dest_name};#{vert_map_id};;#{tecan_precision_value(volume)}
    TECAN

    groups[channel] << step
  end

  blocks = groups.keys.sort.map do |channel|
    # Add 'Comment' between steps, append 'Wash', and join lines.
    (intersperse(groups[channel], 'C;') << 'W;').join("\n")
  end
  blocks.join("\n")
end